30. Jun. 2011, 16:15 Uhr, Gebäude NW1, Raum H3
Deciphering Nature's Molecular Machinery (Gemeinsames Kolloquium Physik/Chemie)
Prof. Dr. Thorsten Hugel, TU München, Dept. Biophysics and Institute for Medical Engineering
Single molecule methods allow real time observation of chemical and biological molecular machines at work. Results of such experiments yield new insights into problems from fundamental chemistry at the nanoscale to biophysical design principles of molecular machines to the development of new materials.
In my presentation I will show how single molecule methods like FRET and Magnetic Tweezers allow deciphering the mechano-chemical cycle of the molecular chaperone and heat shock protein Hsp90. Then I will introduce an AFM-based molecular force sensor (a single polymer grabbed at one end by the AFM tip), which reports on molecular adhesion and friction at surfaces. The following examples show how this force sensor allows for new insights into hydrophobic attraction, co-solute binding to polymers and surface induced structure formation.