24. Jan. 2013, 16:15 Uhr, Gebäude NW1, Raum H3
The physics of gene carriers - gene expression and gene transfer dynamics measured by time-lapse studies
Prof. Dr. Joachim Rädler, Faculty of Physics and Center for NanoScience, Ludwig-Maximilians-University Munich Geschwister-Scholl-Platz 1, D-80539 München, Germany
An ambitious goal in nanoscience is the vision of artificial assemblies with nanoscale architecture, which operate at the molecular level and perform specific functions at a cellular level or within a cellular system, in short: nanoagents. Delivery of exogenous nucleic acid to eukaryotic cells is a prerequisite for DNA based agents. We report on the dynamics and stochastic aspects in artificial gene delivery. Using single cell expression analysis the distribution of onset times, mRNA/eGFP life times and eGFP expression rates is determined. We find mRNA-mediated expression to be in quantitative agreement with mathematical modeling in numbers of successfully delivered and translated mRNA molecules. In this context we also present newly developed mono-molecular siRNA carriers and parallelized cell assays for high-throughput studies and will discuss the control of small groups of cells in defined microenvironments.